新丰江数字遥测台网系统集成与定位校验

新丰江数字遥测地震台网是为了监测和研究河源市新丰江水库区地震活动而建立的区域地震台网，由于在台网数字化改造期间缺少了对台网定位精度的校验，采用人工爆破记录分析对比的方法，对新丰江台网的定位精度进行系统的校验。     

淮南市淮河水域水源保护与防治对策

根据淮河水环境的特点，对水域现状和污染源现状进行了分析，通过对水源保护功能的划分，提出了防治对策和综合整治措施。     

Evaluating and Ranking the Vulnerability of Global Marine Ecosystems to Anthropogenic Threats

Abstract:  Marine ecosystems are threatened by a suite of anthropogenic stressors. Mitigating multiple threats is a daunting task, particularly when funding constraints limit the number of threats that can be addressed. Threats are typically assessed and prioritized via expert opinion workshops that often leave no record of the rationale for decisions, making it difficult to update recommendations with new information. We devised a transparent, repeatable, and modifiable method for collecting expert opinion that describes and documents how threats affect marine ecosystems. Experts were asked to assess the functional impact, scale, and frequency of a threat to an ecosystem; the resistance and recovery time of an ecosystem to a threat; and the certainty of these estimates. To quantify impacts of 38 distinct anthropogenic threats on 23 marine ecosystems, we surveyed 135 experts from 19 different countries. Survey results showed that all ecosystems are threatened by at least nine threats and that nine ecosystems are threatened by > 90% of existing threats. The greatest threats (highest impact scores) were increasing sea temperature, demersal destructive fishing, and point-source organic pollution. Rocky reef, coral reef, hard-shelf, mangrove, and offshore epipelagic ecosystems were identified as the most threatened. These general results, however, may be partly influenced by the specific expertise and geography of respondents, and should be interpreted with caution. This approach to threat analysis can identify the greatest threats (globally or locally), most widespread threats, most (or least) sensitive ecosystems, most (or least) threatened ecosystems, and other metrics of conservation value. Additionally, it can be easily modified, updated as new data become available, and scaled to local or regional settings, which would facilitate informed and transparent conservation priority setting.     

植物生物技术在黑麦草遗传改良中的应用

黑麦草是重要的牧草和草坪草,是畜牧业和草坪业重要的产业支柱.由于高度自交不亲和,传统遗传改良困难而复杂.生物技术尤其是转基因技术为其遗传改良带来希望.本文概述了组织培养技术、基因遗传转化技术、分子标记及数量性状基因定位、分子标记辅助育种等技术在黑麦草遗传改良中的应用及获得的成果;评述了遗传改良的重要意义,以及黑麦草遗传改良的主要目标,如品质改良、提高病害抗性、降低毒性生物碱浓度、控制花粉致敏性、抗除草剂品种的培育、提高抗逆性及其作为生物反应器的潜在应用价值等;并分析了功能基因组学的研究及RNA干扰技术在黑麦草研究中可能的应用前景.     

Avian Diversity and Functional Insectivory on North-Central Florida Farmlands

Abstract:  We studied the potential for native birds to control insect pests on farms. We assessed habitat factors correlated with diversity, distribution, and insect-foraging activity of native birds on farms in north-central Florida and then characterized common bird species that consumed insect biomass in crops as "functional insectivores" (birds most likely to contribute to pest control). Analyses of point-count survey data and foraging observations collected over 2 years on paired organic and conventional farm sites indicated that (1) farms supported most (82–96%) land birds known to breed in the region; (2) bird species richness and abundance varied significantly with matrix habitat and field border type (but not with year or farm management type); (3) the highest bird abundances were associated with mixed crop plantings, field borders, and adjacent matrix composed of forest and hedge; and (4) abundances of 10 species identified as functional insectivores were primarily influenced by crop type (mixed crops attracted significantly more insect foragers into fields than monocrops). We documented birds eating pest insects in crops and did not observe substantive crop damage by birds during growing-season observations. We advocate use of the term functional insectivore to emphasize the potential positive role of avian insectivory on farms during the growing season.     

中药青蒿鲨烯合酶的大肠杆菌表达、纯化与功能鉴定

利用PCR方法,将经RACE方法克隆到的中药青蒿鲨烯合酶cDNA(AF302464)开放阅读框的3′末端截短99bp,插入到原核表达载体pET30a( )的NcoⅠ和BamHⅠ酶切位点之间,构建N端和C端均携带有HIS6表达标签的鲨烯合酶重组表达载体pETSSA.将pETSSA转入大肠杆菌BL21(DE3),0.5mmol/LIPTG(isopropyl-beta-D-thiogalactoside)28℃诱导重组鲨烯合酶的表达.表达产物经镍琼脂糖柱纯化.纯化蛋白加入酶促反应体系(含FPP和NADPH),GC-MS分析酶促反应体系的正己烷萃取物,结果显示重组鲨烯合酶可以催化FPP向鲨烯的转化.青蒿鲨烯合酶的功能鉴定为进一步利用反义或RNAi技术限制甾类生物合成,从而提高青蒿中的青蒿素含量提供了基础.图5参15     

曲靖市环境噪声达标规划

对曲靖市城区进行了环境噪声功能区划分;分析了曲靖市噪声污染现状和趋势、噪声污染原因,对噪声污染结果进行了预测;分析了环境噪声达标规划,提出了噪声污染控制方案。     

异龙湖流域生态功能区划分析

为了改善异龙湖的生态环境，延缓其老化和消亡过程，实现其自然资源的持续利用，以生态学与系统工程学原理为基础，分析异龙湖湖泊生态系统的组成与结构，依据其生态功能分区的原则，将异龙湖流域生态系统划分为4个子系统，分别根据各自的生态功能和主要环境问题，制订有效的保护措施。     

上海市生态环境功能区划综合评价结果

根据上海生态功能区划方案,划分得到8个生态功能区。为了更为清晰、直观地了解各功能区的生态特征,同时也为了便于进行各功能区之间的比较,开展了生态环境敏感性和生态服务功能重要性的综合评价。根据综合评价结果,黄浦江水源保护生态功能区是生态敏感性最强的区域,而长兴岛功能区的生态敏感性最弱;黄浦江水源保护生态功能区是生态服务功能重要性最高的区域,而长兴岛功能区的生态服务功能重要性最弱。     

Heterogeneity of soil surface ammonium concentration and other characteristics, related to plant specific variability in a Mediterranean-type ecosystem

Heterogeneity and dynamics of eight soil surface characteristics essential for plants—ammonium and nitrate concentrations, water content, temperature, pH, organic matter, nitrification and ammonification rates—were studied in a Mediterranean-type ecosystem on four occasions over a year. Soil properties varied seasonally and were influenced by plant species. Nitrate and ammonium were present in the soil at similar concentrations throughout the year. The positive correlation between them at the time of greatest plant development indicates that ammonium is a readily available nitrogen source in Mediterranean-type ecosystems. The results presented here suggest that plant cover significantly affects soil surface characteristics.